An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Integrative analysis of kinase networks in TRAIL-induced apoptosis provides a source of potential targets for combination therapy.

So J, Pasculescu A, Dai AY, Williton K, James A, Nguyen V, Creixell P, Schoof EM, Sinclair J, Barrios-Rodiles M, Gu J, Krizus A, Williams R, Olhovsky M, Dennis JW, Wrana JL, Linding R, Jorgensen C, Pawson T, Colwill K

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is an endogenous secreted peptide and, in preclinical studies, preferentially induces apoptosis in tumor cells rather than in normal cells. The acquisition of resistance in cells exposed to TRAIL or its mimics limits their clinical efficacy. Because kinases are intimately involved in the regulation of apoptosis, we systematically characterized kinases involved in TRAIL signaling. ... [more]

Sci Signal Apr. 09, 2015; 8(371);rs3 [Pubmed: 25852190]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PRKCB SHCBP1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Buljan M (2020)	High	68	BioGRID	3481174

Curated By

BioGRID

Interaction Summary

PRKCB

Gene Ontology Biological Process

Gene Ontology Molecular Function

androgen receptor binding [IDA]chromatin binding [IDA]histone binding [IDA]histone kinase activity (H3-T6 specific) [IDA]ligand-dependent nuclear receptor transcription coactivator activity [IMP]protein binding [IPI]protein kinase C binding [IPI]protein serine/threonine kinase activity [TAS]

Gene Ontology Cellular Component

SHCBP1