An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.

Publication

bHLH05 is an interaction partner of MYB51 and a novel regulator of glucosinolate biosynthesis in Arabidopsis.

Frerigmann H, Berger B, Gigolashvili T

By means of yeast (Saccharomyces cerevisiae) two-hybrid screening, we identified basic helix-loop-helix transcription factor05 (bHLH05) as an interacting partner of MYB51, the key regulator of indolic glucosinolates (GSLs) in Arabidopsis (Arabidopsis thaliana). Furthermore, we show that bHLH04, bHLH05, and bHLH06/MYC2 also interact with other R2R3-MYBs regulating GSL biosynthesis. Analysis of bhlh loss-of-function mutants revealed that the single bhlh mutants retained ... [more]

Plant Physiol. Sep. 01, 2014; 166(1);349-69 [Pubmed: 25049362]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
JAZ1 AT5G46760	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Fernandez-Calvo P (2011)	Low	-	BioGRID	-
JAZ1 AT5G46760	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Niu Y (2011)	Low	-	BioGRID	-
AT5G46760 JAZ1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Fernandez-Calvo P (2011)	Low	-	BioGRID	-
JAZ1 AT5G46760	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Fernandez-Calvo P (2011)	Low	-	BioGRID	-
JAZ1 AT5G46760	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Goossens J (2015)	Low	-	BioGRID	-
JAZ1 AT5G46760	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Niu Y (2011)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

AT5G46760

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [ISS]bHLH transcription factor binding [IPI]protein binding [IPI]sequence-specific DNA binding transcription factor activity [ISS]

Gene Ontology Cellular Component

JAZ1