Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The Arabidopsis bHLH Transcription Factors MYC3 and MYC4 Are Targets of JAZ Repressors and Act Additively with MYC2 in the Activation of Jasmonate Responses.

Fernandez-Calvo P, Chini A, Fernandez-Barbero G, Chico JM, Gimenez-Ibanez S, Geerinck J, Eeckhout D, Schweizer F, Godoy M, Franco-Zorrilla JM, Pauwels L, Witters E, Puga MI, Paz-Ares J, Goossens A, Reymond P, De Jaeger G, Solano R

Jasmonates (JAs) trigger an important transcriptional reprogramming of plant cells to modulate both basal development and stress responses. In spite of the importance of transcriptional regulation, only one transcription factor (TF), the Arabidopsis thaliana basic helix-loop-helix MYC2, has been described so far as a direct target of JAZ repressors. By means of yeast two-hybrid screening and tandem affinity purification strategies, ... [more]

Plant Cell Feb. 01, 2011; 23(2);701-15 [Pubmed: 21335373]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AT5G46760 JAZ1	Affinity Capture-Luminescence Affinity Capture-Luminescence An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.	Frerigmann H (2014)	Low	-	BioGRID	-
JAZ1 AT5G46760	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Fernandez-Calvo P (2011)	Low	-	BioGRID	-
JAZ1 AT5G46760	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Niu Y (2011)	Low	-	BioGRID	-
JAZ1 AT5G46760	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Fernandez-Calvo P (2011)	Low	-	BioGRID	-
JAZ1 AT5G46760	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Goossens J (2015)	Low	-	BioGRID	-
JAZ1 AT5G46760	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Niu Y (2011)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

AT5G46760

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [ISS]bHLH transcription factor binding [IPI]protein binding [IPI]sequence-specific DNA binding transcription factor activity [ISS]

Gene Ontology Cellular Component

JAZ1