BAIT

BRI1

ATBRI1, BIN1, BR INSENSITIVE 1, BRASSINOSTEROID INSENSITIVE 1, CABBAGE 2, CBB2, DWARF 2, DWF2, F23K16.30, F23K16_30, AT4G39400
protein brassinosteroid insensitive 1
Arabidopsis thaliana (Columbia)
PREY

VAM3

ARABIDOPSIS THALIANA SYNTAXIN OF PLANTS 22, ARABIDOPSIS THALIANA VACUOLAR MORPHOLOGY 3, ATSYP22, ATVAM3, SGR3, SHOOT GRAVITROPISM 3, SYNTAXIN OF PLANTS 22, SYP22, VACUOLAR MORPHOLOGY 3, AT5G46860
syntaxin-22
Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Border control--a membrane-linked interactome of Arabidopsis.

Jones AM, Xuan Y, Xu M, Wang RS, Ho CH, Lalonde S, You CH, Sardi MI, Parsa SA, Smith-Valle E, Su T, Frazer KA, Pilot G, Pratelli R, Grossmann G, Acharya BR, Hu HC, Engineer C, Villiers F, Ju C, Takeda K, Su Z, Dong Q, Assmann SM, Chen J, Kwak JM, Schroeder JI, Albert R, Rhee SY, Frommer WB

Cellular membranes act as signaling platforms and control solute transport. Membrane receptors, transporters, and enzymes communicate with intracellular processes through protein-protein interactions. Using a split-ubiquitin yeast two-hybrid screen that covers a test-space of 6.4 × 10(6) pairs, we identified 12,102 membrane/signaling protein interactions from Arabidopsis. Besides confirmation of expected interactions such as heterotrimeric G protein subunit interactions and aquaporin oligomerization, ... [more]

Science May. 16, 2014; 344(6185);711-6 [Pubmed: 24833385]

Throughput

  • Low Throughput

Curated By

  • BioGRID