BAIT

CMR1

YDL156W

DNA-binding protein with preference for UV-damaged DNA; protein sequence contains three WD domains (WD-40 repeat); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; potential regulatory target of Mbp1p, which binds to the promoter region; co-localizes with Hos2p in nuclear foci in response to DNA damage by MMS

GO Process (0)

GO Function (1)

GO Component (3)

Gene Ontology Molecular Function

DNA binding [IDA]

Gene Ontology Cellular Component

cytoplasm [IDA]

nuclear chromatin [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

EMI1

YDR512C

Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs

GO Process (3)

GO Function (0)

GO Component (0)

Gene Ontology Biological Process

ascospore formation [IMP]

mitochondrial respiratory chain complex assembly [IMP]

positive regulation of transcription from RNA polymerase II promoter [IMP]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Cmr1/WDR76 defines a nuclear genotoxic stress body linking genome integrity and protein quality control.

Gallina I, Colding C, Henriksen P, Beli P, Nakamura K, Offman J, Mathiasen DP, Silva S, Hoffmann E, Groth A, Choudhary C, Lisby M

DNA replication stress is a source of genomic instability. Here we identify changed mutation rate 1 (Cmr1) as a factor involved in the response to DNA replication stress in Saccharomyces cerevisiae and show that Cmr1-together with Mrc1/Claspin, Pph3, the chaperonin containing TCP1 (CCT) and 25 other proteins-define a novel intranuclear quality control compartment (INQ) that sequesters misfolded, ubiquitylated and sumoylated ... [more]

Nat Commun Mar. 31, 2015; 6(0);6533 [Pubmed: 25817432]

Quantitative Score

7.46 [Confidence Score]

Throughput

High Throughput

Additional Notes

SGA performed with cmr1 as bait in the presence of hydroxyurea; cut-off is 0.25 for positive genetic and 1.50 for negative genetic

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CMR1 EMI1	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Tkach JM (2012)	High	-0.127	BioGRID	721946
EMI1 CMR1	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Tkach JM (2012)	High	-0.127	BioGRID	721947

Curated By

BioGRID

Interaction Summary