BAIT

MAX2

F14N22.11, F14N22_11, MORE AXILLARY BRANCHES 2, ORE9, ORESARA 9, PLEIOTROPIC PHOTOSIGNALING, PPS, AT2G42620
F-box leucine-rich repeat family protein MAX2
Arabidopsis thaliana (Columbia)
PREY

BES1

107 PROTEIN, BRASSINAZOLE-RESISTANT 2, BRI1-EMS-SUPPRESSOR 1, BZR2, F18O14.7, F18O14_7, AT1G19350
protein brassinazole-resistant 2
GO Process (2)
GO Function (3)
GO Component (2)
Arabidopsis thaliana (Columbia)

FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

Strigolactone/MAX2-induced degradation of brassinosteroid transcriptional effector BES1 regulates shoot branching.

Wang Y, Sun S, Zhu W, Jia K, Yang H, Wang X

Strigolactones (SLs), a class of the most recently identified terpenoid phytohormones, play essential roles in plant development, specifically in suppressing shoot branching. MAX2, a subunit of an SCF E3 ligase and a positive regulator that inhibits shoot branching, is likely a key SL signaling component. Here, we provide genetic and biochemical evidence to demonstrate that BES1 interacts with MAX2 and ... [more]

Dev. Cell Dec. 23, 2013; 27(6);681-8 [Pubmed: 24369836]

Throughput

  • Low Throughput

Additional Notes

  • BiFC assay

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
BES1 MAX2
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
1116000
MAX2 BES1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID