BAIT
ERG11
CYP51, sterol 14-demethylase, L000000577, YHR007C
Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
GO Process (1)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
BRE1
E3 ubiquitin-protein ligase BRE1, YDL074C
E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control; interacts with RNA-binding protein Npl3p, linking histone ubiquitination to mRNA processing; Bre1p-dependent histone ubiquitination promotes pre-mRNA splicing
GO Process (10)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
- chromatin silencing at telomere [IMP]
- double-strand break repair via homologous recombination [IGI]
- histone monoubiquitination [IMP]
- histone ubiquitination [IMP]
- intra-S DNA damage checkpoint [IMP]
- meiotic DNA double-strand break formation [IMP]
- mitotic G1 DNA damage checkpoint [IMP]
- regulation of DNA-dependent DNA replication initiation [IMP]
- telomere maintenance via recombination [IGI]
- transcription from RNA polymerase II promoter [IGI, IPI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Integration of chemical-genetic and genetic interaction data links bioactive compounds to cellular target pathways.
Bioactive compounds can be valuable research tools and drug leads, but it is often difficult to identify their mechanism of action or cellular target. Here we investigate the potential for integration of chemical-genetic and genetic interaction data to reveal information about the pathways and targets of inhibitory compounds. Taking advantage of the existing complete set of yeast haploid deletion mutants, ... [more]
Nat. Biotechnol. Jan. 01, 2004; 22(1);62-9 [Pubmed: 14661025]
Throughput
- High Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Curated By
- BioGRID