KIF23
Gene Ontology Biological Process
- ATP catabolic process [IBA]
- antigen processing and presentation of exogenous peptide antigen via MHC class II [TAS]
- blood coagulation [TAS]
- cytokinesis [IMP]
- metabolic process [IBA, TAS]
- microtubule-based movement [IBA, TAS]
- mitotic cell cycle [TAS]
- mitotic cytokinesis [IMP]
- mitotic spindle elongation [TAS]
- mitotic spindle midzone assembly [IMP]
- positive regulation of cytokinesis [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ARF6
Gene Ontology Biological Process
- GTP catabolic process [TAS]
- cortical actin cytoskeleton organization [ISO]
- establishment of epithelial cell polarity [IMP]
- hepatocyte apoptotic process [IMP]
- liver development [IMP]
- myeloid cell apoptotic process [IMP]
- negative regulation of dendrite development [ISO]
- positive regulation of actin filament polymerization [ISO]
- positive regulation of establishment of protein localization to plasma membrane [IDA]
- protein localization to cell surface [IDA]
- protein localization to endosome [IMP, ISO]
- protein transport [TAS]
- regulation of Rac protein signal transduction [ISO]
- regulation of dendritic spine development [IMP, ISO]
- regulation of filopodium assembly [ISO]
- regulation of toll-like receptor 4 signaling pathway [NAS]
- ruffle organization [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cell cortex [ISO]
- cytoplasm [IDA]
- early endosome [ISO]
- endocytic vesicle [ISO]
- endosome [IDA, ISO, TAS]
- extracellular vesicular exosome [ISO]
- filopodium membrane [ISO]
- focal adhesion [ISO]
- membrane [ISO]
- myelin sheath [IDA]
- plasma membrane [IDA, ISO]
- recycling endosome [ISO]
- recycling endosome membrane [ISO]
- ruffle [ISO]
Co-crystal Structure
Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.
Publication
Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis.
A small GTPase, Arf6, is involved in cytokinesis by localizing to the Flemming body (the midbody). However, it remains unknown how Arf6 contributes to cytokinesis. Here, we demonstrate that Arf6 directly interacts with mitotic kinesin-like protein 1 (MKLP1), a Flemming body-localizing protein essential for cytokinesis. The crystal structure of the Arf6-MKLP1 complex reveals that MKLP1 forms a homodimer flanked by ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| KIF23 ARF6 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID