SPHK1
Gene Ontology Biological Process
- 'de novo' posttranslational protein folding [TAS]
- calcium-mediated signaling [IDA]
- cellular protein metabolic process [TAS]
- intracellular signal transduction [TAS]
- lipid phosphorylation [IDA]
- negative regulation of apoptotic process [IDA, TAS]
- positive regulation of NF-kappaB import into nucleus [IMP]
- positive regulation of NF-kappaB transcription factor activity [IMP]
- positive regulation of angiogenesis [IDA]
- positive regulation of cell growth [IDA]
- positive regulation of cell migration [IDA]
- positive regulation of fibroblast proliferation [IDA]
- positive regulation of mitotic cell cycle [IDA]
- positive regulation of protein ubiquitination [IDA]
- positive regulation of smooth muscle contraction [IDA]
- protein folding [TAS]
- regulation of tumor necrosis factor-mediated signaling pathway [IDA]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
- sphingoid catabolic process [NAS]
- sphingolipid biosynthetic process [TAS]
- sphingolipid metabolic process [TAS]
- sphingosine metabolic process [IDA]
Gene Ontology Molecular Function
CYLD
Gene Ontology Biological Process
- cytoplasmic translation [IBA]
- innate immune response [TAS]
- necroptotic process [IBA]
- negative regulation of NF-kappaB import into nucleus [IDA]
- negative regulation of NF-kappaB transcription factor activity [IDA]
- negative regulation of canonical Wnt signaling pathway [IMP]
- negative regulation of type I interferon production [TAS]
- nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway [TAS]
- nucleotide-binding oligomerization domain containing signaling pathway [TAS]
- positive regulation of extrinsic apoptotic signaling pathway [IMP]
- protein K63-linked deubiquitination [IDA]
- regulation of cilium assembly [ISS]
- regulation of intrinsic apoptotic signaling pathway [IMP]
- regulation of microtubule cytoskeleton organization [IMP]
- regulation of mitotic cell cycle [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The BioPlex Network: A Systematic Exploration of the Human Interactome.
Protein interactions form a network whose structure drives cellular function and whose organization informs biological inquiry. Using high-throughput affinity-purification mass spectrometry, we identify interacting partners for 2,594 human proteins in HEK293T cells. The resulting network (BioPlex) contains 23,744 interactions among 7,668 proteins with 86% previously undocumented. BioPlex accurately depicts known complexes, attaining 80%-100% coverage for most CORUM complexes. The network ... [more]
Quantitative Score
- 0.999978943 [compPASS Score]
Throughput
- High Throughput
Additional Notes
- BioPlex 1.0 HEK 293T cells CompPASS score = 0.999978943, threshold = 0.75. Quantitative scores calculated by CompPASS-Plus (Huttlin et al. Cell, 2015, PMID: 26186194).
- See BioPlex Interactome for details (https://bioplex.hms.harvard.edu/index.php).
- This data has also been reanalyzed as part of BioPlex 2.0 (PMID: 28514442) and BioPlex 3.0 (PMID: 33961781). Only scores from within BioPlex 1.0 (PMID: 26186194) should be compared directly.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SPHK1 CYLD | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 2221883 | |
| SPHK1 CYLD | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9969 | BioGRID | 3181889 |
Curated By
- BioGRID