BAIT

HCA4

DBP4, ECM24, RNA-dependent ATPase HCA4, L000003421, YJL033W

DEAD box RNA helicase; component of the SSU; interacts with Bfr2p and Enp2p; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis

GO Process (1)

GO Function (2)

GO Component (2)

Gene Ontology Biological Process

rRNA processing [IMP]

Gene Ontology Molecular Function

ATP-dependent RNA helicase activity [IDA, IMP]
RNA-dependent ATPase activity [IDA]

Gene Ontology Cellular Component

nucleolus [IC]

small-subunit processome [IMP]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SNR17A

U3, L000001966

Small nucleolar RNA (snoRNA) U3; part of Small ribosomal SubUnit (SSU) processosome; SNR17B also encodes snoRNA U3

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene

Saccharomyces cerevisiae (S288c)

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Nucleolar proteins Bfr2 and Enp2 interact with DEAD-box RNA helicase Dbp4 in two different complexes.

Soltanieh S, Lapensee M, Dragon F

Different pre-ribosomal complexes are formed during ribosome biogenesis, and the composition of these complexes is highly dynamic. Dbp4, a conserved DEAD-box RNA helicase implicated in ribosome biogenesis, interacts with nucleolar proteins Bfr2 and Enp2. We show that, like Dbp4, Bfr2 and Enp2 are required for the early processing steps leading to the production of 18S ribosomal RNA. We also found ... [more]

Nucleic Acids Res. Mar. 01, 2014; 42(5);3194-206 [Pubmed: 24357410]

Throughput

Low Throughput

Additional Notes

Figure 5

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HCA4 SNR17A	Affinity Capture-RNA Affinity Capture-RNA An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.	Soltanieh S (2015)	Low	-	BioGRID	1504320

Curated By

BioGRID

Interaction Summary