BAIT

HCA4

DBP4, ECM24, RNA-dependent ATPase HCA4, L000003421, YJL033W

DEAD box RNA helicase; component of the SSU; interacts with Bfr2p and Enp2p; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis

GO Process (1)

GO Function (2)

GO Component (2)

Gene Ontology Biological Process

rRNA processing [IMP]

Gene Ontology Molecular Function

ATP-dependent RNA helicase activity [IDA, IMP]
RNA-dependent ATPase activity [IDA]

Gene Ontology Cellular Component

nucleolus [IC]

small-subunit processome [IMP]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SNR17A

U3, L000001966

Small nucleolar RNA (snoRNA) U3; part of Small ribosomal SubUnit (SSU) processosome; SNR17B also encodes snoRNA U3

GO Process (0)

GO Function (0)

GO Component (0)

SGD Alliance of Genome Resources Entrez Gene

Saccharomyces cerevisiae (S288c)

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

DEAD-box RNA helicase Dbp4 is required for small-subunit processome formation and function.

Soltanieh S, Osheim YN, Spasov K, Trahan C, Beyer AL, Dragon F

DEAD-box RNA helicase Dbp4 is required for 18S rRNA synthesis: cellular depletion of Dbp4 impairs the early cleavage reactions of the pre-rRNA and causes U14 small nucleolar RNA (snoRNA) to remain associated with pre-rRNA. Immunoprecipitation experiments (IPs) carried out with whole-cell extracts (WCEs) revealed that hemagglutinin (HA)-tagged Dbp4 is associated with U3 snoRNA but not with U14 snoRNA. IPs with ... [more]

Mol. Cell. Biol. Mar. 01, 2015; 35(5);816-30 [Pubmed: 25535329]

Throughput

Low Throughput

Additional Notes

Figure 1

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HCA4 SNR17A	Affinity Capture-RNA Affinity Capture-RNA An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.	Soltanieh S (2014)	Low	-	BioGRID	1239874

Curated By

BioGRID

Interaction Summary