BAIT

CIK1

L000000335, YMR198W
Kinesin-associated protein; required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; locus encodes a long and short transcript with differing functions; CIK1 has a paralog, VIK1, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

IPL1

PAC15, aurora kinase, L000000871, YPL209C
Aurora kinase of conserved chromosomal passenger complex; mediates release on mono-oriented kinetochores from microtubules in meiosis I, also release of kinetochores from cluster at SPBs at meiosis exit; helps maintain condensed chromosomes during anaphase, early telophase; required for SPB cohesion and prevention of multipolar spindle formation; Iocalizes to nuclear foci that diffuse upon DNA replication stress; required for inhibition of karyopherin Pse1p upon SAC arrest
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

The signaling network that silences the spindle assembly checkpoint upon the establishment of chromosome bipolar attachment.

Jin F, Wang Y

Improper kinetochore attachments activate the spindle assembly checkpoint (SAC) to prevent anaphase onset, but it is poorly understood how this checkpoint is silenced to allow anaphase onset. Chromosome bipolar attachment applies tension on sister kinetochores, and the lack of tension delays anaphase onset. In budding yeast, the delay induced by tension defects depends on the intact SAC as well as ... [more]

Proc. Natl. Acad. Sci. U.S.A. Dec. 24, 2013; 110(52);21036-41 [Pubmed: 24324173]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: cell size (APO:0000052)
  • phenotype: protein/peptide modification (APO:0000131)

Additional Notes

  • Figure 1
  • ipl1-321 mutation suppresses increase in large budded cells caused by overexpression of Cik1 coiled-coil domain
  • ipl1-321 mutation suppresses persistant Mad1 phosphorylation caused by overexpression of Cik1 coiled-coil domain

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CIK1 IPL1
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
632074

Curated By

  • BioGRID