BAIT

PRE6

proteasome core particle subunit alpha 4, L000001488, YOL038W
Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
Saccharomyces cerevisiae (S288c)
PREY

IRC25

DMP2, PBA3, POC3, YLR021W
Component of a heterodimeric Poc4p-Irc25p chaperone; involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
GO Process (3)
GO Function (0)
GO Component (1)
Saccharomyces cerevisiae (S288c)

Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Publication

Pba3-Pba4 heterodimer acts as a molecular matchmaker in proteasome α-ring formation.

Takagi K, Saeki Y, Yashiroda H, Yagi H, Kaiho A, Murata S, Yamane T, Tanaka K, Mizushima T, Kato K

Eukaryotic proteasome assembly is assisted by multiple dedicated chaperones. In yeast, formation of the heteroheptameric ring composed of α1-α7 subunits is promoted by the heterodimeric chaperone Pba3-Pba4. Here we reveal that in the absence of this dimeric chaperone, α2 replaces α4 during α-ring assembly, thereby giving rise to a non-productive complex that lacks α4, β1, β5, β6, and β7 subunits ... [more]

Biochem. Biophys. Res. Commun. Jul. 25, 2014; 450(2);1110-4 [Pubmed: 24996173]

Throughput

  • Low Throughput

Additional Notes

  • Figure 1

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
IRC25 PRE6
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.4929BioGRID
2056208
PRE6 IRC25
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.7692BioGRID
2014165
IRC25 PRE6
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.8064BioGRID
2438761
IRC25 PRE6
Synthetic Rescue
Synthetic Rescue

A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.

Low-BioGRID
346222

Curated By

  • BioGRID