PIN7
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
AT1G34640
Gene Ontology Cellular Component
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
Border control--a membrane-linked interactome of Arabidopsis.
Cellular membranes act as signaling platforms and control solute transport. Membrane receptors, transporters, and enzymes communicate with intracellular processes through protein-protein interactions. Using a split-ubiquitin yeast two-hybrid screen that covers a test-space of 6.4 × 10(6) pairs, we identified 12,102 membrane/signaling protein interactions from Arabidopsis. Besides confirmation of expected interactions such as heterotrimeric G protein subunit interactions and aquaporin oligomerization, ... [more]
Throughput
- High Throughput
Additional Notes
- A split-ubiquitin screen identified over 12,000 interactions between membrane proteins and signaling proteins in Arabidopsis thaliana. The results are also provided in the Membrane-based Interactome Database (MIND1).
Curated By
- BioGRID