BAIT

DNF2

aminophospholipid-translocating P4-type ATPase DNF2, YDR093W
Aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to endocytosis, protein transport, and cellular polarization; localizes primarily to the plasma membrane; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone; DNF2 has a paralog, DNF1, that arose from the whole genome duplication
Saccharomyces cerevisiae (S288c)
PREY

CLN1

cyclin CLN1, L000000357, YMR199W
G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p); CLN1 has a paralog, CLN2, that arose from the whole genome duplication
Kinome Project (Sc)
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Plasma membrane aminoglycerolipid flippase function is required for signaling competence in the yeast mating pheromone response pathway.

Sartorel E, Barrey E, Lau RK, Thorner J

The class 4 P-type ATPases ("flippases") maintain membrane asymmetry by translocating phosphatidylethanolamine and phosphatidylserine from the outer leaflet to the cytosolic leaflet of the plasma membrane. In Saccharomyces cerevisiae, five related gene products (Dnf1, Dnf2, Dnf3, Drs2, and Neo1) are implicated in flipping of phosphatidylethanolamine, phosphatidylserine, and phosphatidylcholine. In MAT A: cells responding to α-factor, we found that Dnf1, Dnf2, ... [more]

Mol. Biol. Cell Jan. 01, 2015; 26(1);134-50 [Pubmed: 25378585]

Throughput

  • Low Throughput

Ontology Terms

  • shmoo formation (APO:0000038)
  • protein/peptide accumulation (APO:0000149)

Additional Notes

  • CLN1 and CLN3 double deletion partially rescues Ste5 levels and shmoo formation in dnf1 dnf2 dnf3 triple mutant
  • Figure 5
  • genetic complex

Curated By

  • BioGRID