BAIT

UBP15

L000004589, YMR304W
Ubiquitin-specific protease involved in protein deubiquitination; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; forms a complex with AAA peroxins Pex1p and Pex6p
UPS Project
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

CLB5

B-type cyclin CLB5, L000000353, YPR120C
B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase; CLB5 has a paralog, CLB6, that arose from the whole genome duplication
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

The Ubp15 deubiquitinase promotes timely entry into S phase in Saccharomyces cerevisiae.

Ostapenko D, Burton JL, Solomon MJ

The anaphase-promoting complex in partnership with its activator, Cdh1, is an E3 ubiquitin ligase responsible for targeting cell cycle proteins during G1 phase. In the budding yeast Saccharomyces cerevisiae, Cdh1 associates with the deubiquitinating enzyme Ubp15, but the significance of this interaction is unclear. To better understand the physiological role(s) of Ubp15, we examined cell cycle phenotypes of cells lacking ... [more]

Mol. Biol. Cell Jun. 15, 2015; 26(12);2205-16 [Pubmed: 25877870]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: cell cycle progression (APO:0000253)
  • phenotype: budding index (APO:0000089)

Additional Notes

  • CLB5 overexpression rescues budding index defect in ubp15 mutant
  • CLB5 overexpression restores cell cycle progression in ubp15 mutant
  • Figure 1
  • Figure S3

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
UBP15 CLB5
Dosage Rescue
Dosage Rescue

A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.

Low-BioGRID
1255291
UBP15 CLB5
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
1255301

Curated By

  • BioGRID