BAIT

ZMYND11

BRAM1, BS69, MRD30, RP11-486H9.1
zinc finger, MYND-type containing 11
UPS Project
Homo sapiens
PREY

SNRNP200

ASCC3L1, BRR2, HELIC2, RP33, U5-200KD
small nuclear ribonucleoprotein 200kDa (U5)
Cell Cycle - Mitotic Spindle Assembly Project
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

BS69/ZMYND11 reads and connects histone H3.3 lysine 36 trimethylation-decorated chromatin to regulated pre-mRNA processing.

Guo R, Zheng L, Park JW, Lv R, Chen H, Jiao F, Xu W, Mu S, Wen H, Qiu J, Wang Z, Yang P, Wu F, Hui J, Fu X, Shi X, Shi YG, Xing Y, Lan F, Shi Y

BS69 (also called ZMYND11) contains tandemly arranged PHD, BROMO, and PWWP domains, which are chromatin recognition modalities. Here, we show that BS69 selectively recognizes histone variant H3.3 lysine 36 trimethylation (H3.3K36me3) via its chromatin-binding domains. We further identify BS69 association with RNA splicing regulators, including the U5 snRNP components of the spliceosome, such as EFTUD2. Remarkably, RNA sequencing shows that ... [more]

Mol. Cell Oct. 23, 2014; 56(2);298-310 [Pubmed: 25263594]

Throughput

  • High Throughput

Curated By

  • BioGRID