BAIT

ATG1

APG1, AUT3, CVT10, serine/threonine protein kinase ATG1, L000003955, S000028502, L000004761, YGL180W
Protein serine/threonine kinase; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p; essential for cell cycle progression from G2/M to G1 under nitrogen starvation
Kinome Project (Sc)
Saccharomyces cerevisiae (S288c)
PREY

ORC3

origin recognition complex subunit 3, L000003074, YLL004W
Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
Saccharomyces cerevisiae (S288c)

Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

Autophagy competes for a common phosphatidylethanolamine pool with major cellular PE-consuming pathways in Saccharomyces cerevisiae.

Wilson-Zbinden C, dos Santos AX, Stoffel-Studer I, van der Vaart A, Hofmann K, Reggiori F, Riezman H, Kraft C, Peter M

Autophagy is a highly regulated pathway that selectively degrades cellular constituents such as protein aggregates and excessive or damaged organelles. This transport route is characterized by engulfment of the targeted cargo by autophagosomes. The formation of these double-membrane vesicles requires the covalent conjugation of the ubiquitin-like protein Atg8 to phosphatidylethanolamine (PE). However, the origin of PE and the regulation of ... [more]

Genetics Feb. 01, 2015; 199(2);475-85 [Pubmed: 25519895]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: inviable (APO:0000112)

Additional Notes

  • SGA with atg1 mutant as bait
  • Table S1

Curated By

  • BioGRID