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BAIT

AMBRA1

DCAF3, WDR94

autophagy/beclin-1 regulator 1

Autophagy Project

GO Process (4)

GO Function (2)

GO Component (5)

Gene Ontology Biological Process

autophagy [IBA]

positive regulation of mitochondrion degradation [IDA, IGI, IMP]

positive regulation of phosphatidylinositol 3-kinase activity [IDA]

regulation of autophagic vacuole assembly [IMP]

Gene Ontology Molecular Function

protein binding [IPI]
ubiquitin protein ligase binding [IPI]

Gene Ontology Cellular Component

cytoplasm [IDA]

intracellular membrane-bounded organelle [IBA]

mitochondrion [IDA, IMP]

CRISPR Database HGNC Alliance of Genome Resources OMIM VEGA Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

PREY

HSP90AA1

EL52, HSP86, HSP89A, HSP90A, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, Hsp89, Hsp90, LAP-2, LAP2

heat shock protein 90kDa alpha (cytosolic), class A member 1

Alzheimer's Disease Project

GO Process (16)

GO Function (10)

GO Component (10)

Gene Ontology Molecular Function

ATP binding [TAS]
ATPase activity [IDA]
MHC class II protein complex binding [IDA]
TPR domain binding [IDA, TAS]
identical protein binding [IPI]
nitric-oxide synthase regulator activity [IDA]
nucleotide binding [TAS]
poly(A) RNA binding [IDA]
protein binding [IPI]
protein homodimerization activity [TAS]

Gene Ontology Cellular Component

cytoplasm [IDA]

cytosol [NAS, TAS]

endocytic vesicle lumen [TAS]

extracellular region [TAS]

extracellular vesicular exosome [IDA]

mitochondrion [IDA]

nucleoplasm [IDA]

plasma membrane [TAS]

CRISPR Database VEGA HGNC Alliance of Genome Resources OMIM Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

AMBRA1 interplay with cullin E3 ubiquitin ligases regulates autophagy dynamics.

Antonioli M, Albiero F, Nazio F, Vescovo T, Perdomo AB, Corazzari M, Marsella C, Piselli P, Gretzmeier C, Dengjel J, Cecconi F, Piacentini M, Fimia GM

Autophagy maintains cellular homeostasis by degrading harmful or unnecessary intracellular components. How the autophagy response is induced rapidly and transiently remains largely unknown. We report that the E3 ubiquitin ligases Cullin-5 and Cullin-4 regulate the onset and termination of autophagy, respectively, by dynamically interacting with AMBRA1, a regulator of autophagy. Under normal conditions, Cullin-4 binding to AMBRA1 limits its protein ... [more]

Dev. Cell Dec. 22, 2014; 31(6);734-46 [Pubmed: 25499913]

Throughput

High Throughput

Curated By

BioGRID