BAIT

PMT1

dolichyl-phosphate-mannose-protein mannosyltransferase PMT1, L000001458, YDL095W
Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen
Saccharomyces cerevisiae (S288c)
PREY

HAC1

ERN4, IRE15, transcription factor HAC1, L000002611, YFL031W
Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates the unfolded protein response, via UPRE binding, and membrane biogenesis; ER stress-induced splicing pathway facilitates efficient Hac1p synthesis; protein abundance increases in response to DNA replication stress
Saccharomyces cerevisiae (S288c)

Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

PMT1 deficiency enhances basal UPR activity and extends replicative lifespan of Saccharomyces cerevisiae.

Cui HJ, Liu XG, McCormick M, Wasko BM, Zhao W, He X, Yuan Y, Fang BX, Sun XR, Kennedy BK, Suh Y, Zhou ZJ, Kaeberlein M, Feng WL

Pmt1p is an important member of the protein O-mannosyltransferase (PMT) family of enzymes, which participates in the endoplasmic reticulum (ER) unfolded protein response (UPR), an important pathway for alleviating ER stress. ER stress and the UPR have been implicated in aging and age-related diseases in several organisms; however, a possible role for PMT1 in determining lifespan has not been previously ... [more]

Age (Dordr) Jun. 01, 2015; 37(3);9788 [Pubmed: 25936926]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: chronological lifespan (APO:0000316)
  • phenotype: rna accumulation (APO:0000224)

Additional Notes

  • Deletion of IRE1 or HAC1 reverses increased lifespan of pmt1 mutant
  • Figure 4

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PMT1 HAC1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-3.4218BioGRID
898367
PMT1 HAC1
Phenotypic Suppression
Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
2575031

Curated By

  • BioGRID