An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

Control of somatic tissue differentiation by the long non-coding RNA TINCR.

Kretz M, Siprashvili Z, Chu C, Webster DE, Zehnder A, Qu K, Lee CS, Flockhart RJ, Groff AF, Chow J, Johnston D, Kim GE, Spitale RC, Flynn RA, Zheng GX, Aiyer S, Raj A, Rinn JL, Chang HY, Khavari PA

Several of the thousands of human long non-coding RNAs (lncRNAs) have been functionally characterized; however, potential roles for lncRNAs in somatic tissue differentiation remain poorly understood. Here we show that a 3.7-kilobase lncRNA, terminal differentiation-induced ncRNA (TINCR), controls human epidermal differentiation by a post-transcriptional mechanism. TINCR is required for high messenger RNA abundance of key differentiation genes, many of which ... [more]

Nature Jan. 10, 2013; 493(7431);231-5 [Pubmed: 23201690]

Throughput

Low Throughput

Additional Notes

RNA pull-down using lncRNA TINCR with subsequent STAU1 protein detection

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
STAU1 TINCR	Protein-RNA Protein-RNA An interaction is detected between and protein and an RNA in vitro.	Kretz M (2013)	Low	-	BioGRID	1257306
TINCR STAU1	Protein-RNA Protein-RNA An interaction is detected between and protein and an RNA in vitro.	Kretz M (2013)	Low	-	BioGRID	1257315

Curated By

BioGRID

Interaction Summary

TINCR

STAU1

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

Affinity Capture-RNA