HRR25
Gene Ontology Biological Process
- DNA repair [IMP]
- ER to Golgi vesicle-mediated transport [IMP]
- attachment of spindle microtubules to kinetochore involved in homologous chromosome segregation [IMP]
- mitotic nuclear division [IMP]
- protein phosphorylation [IDA, IMP]
- ribosomal large subunit biogenesis [IMP]
- ribosomal small subunit biogenesis [IMP]
- tRNA wobble uridine modification [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ELP2
Gene Ontology Biological Process
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
The path from nucleolar 90S to cytoplasmic 40S pre-ribosomes.
Recent reports have increased our knowledge of the consecutive steps during 60S ribosome biogenesis substantially, but 40S subunit formation is less well understood. Here, we investigate the maturation of nucleolar 90S pre-ribosomes into cytoplasmic 40S pre-ribosomes. During the transition from 90S to 40S particles, the majority of non-ribosomal proteins (approximately 30 species) dissociate, and significantly fewer factors associate with 40S ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
HRR25 ELP2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
ELP2 HRR25 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
HRR25 ELP2 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | High | - | BioGRID | 520512 |
Curated By
- BioGRID