SIN4
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
HOG1
Gene Ontology Biological Process
- cellular response to heat [IMP]
- cellular response to osmotic stress [IMP]
- hyperosmotic response [IMP]
- osmosensory signaling pathway [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription from RNA polymerase II promoter in response to osmotic stress [IMP]
- protein phosphorylation [IDA]
- regulation of nuclear cell cycle DNA replication [IDA]
- regulation of transcription from RNA polymerase II promoter in response to osmotic stress [IDA]
- response to arsenic-containing substance [IGI, IMP]
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Osmostress-induced transcription by Hot1 depends on a Hog1-mediated recruitment of the RNA Pol II.
In budding yeast, the mitogen-activated protein kinase (MAPK) Hog1 coordinates the transcriptional program required for cell survival upon osmostress. The Hot1 transcription factor acts downstream of the MAPK and regulates a subset of Hog1-responsive genes. In response to high osmolarity, Hot1 targets Hog1 to specific osmostress-responsive promoters. Here, we show that assembly of the general transcription machinery at Hot1-dependent promoters ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SIN4 HOG1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.3544 | BioGRID | 408543 | |
| SIN4 HOG1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.3547 | BioGRID | 2172597 | |
| SIN4 HOG1 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.3544 | BioGRID | 909915 |
Curated By
- BioGRID