BAIT

TOA1

L000002316, YOR194C

TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; required, along with Toa2p, for ribosomal protein gene transcription in vivo; homologous to largest and second largest subunits of human and Drosophila TFIIA

GO Process (2)

GO Function (2)

GO Component (1)

Gene Ontology Biological Process

RNA polymerase II transcriptional preinitiation complex assembly [IDA, IGI]

transcription initiation from RNA polymerase II promoter [IDA]

Gene Ontology Molecular Function

TBP-class protein binding [IDA]
TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA, IGI]

Gene Ontology Cellular Component

transcription factor TFIIA complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SUA7

SOH4, TFIIB, L000002141, YPR086W

Transcription factor TFIIB; a general transcription factor required for transcription initiation and start site selection by RNA polymerase II

GO Process (4)

GO Function (4)

GO Component (2)

Gene Ontology Biological Process

RNA polymerase II transcriptional preinitiation complex assembly [IDA, IMP]

positive regulation of transcription regulatory region DNA binding [IDA]

transcriptional open complex formation at RNA polymerase II promoter [IMP]

transcriptional start site selection at RNA polymerase II promoter [IMP]

Gene Ontology Molecular Function

RNA polymerase II core binding [IDA, IPI]
TBP-class protein binding [IDA, IPI]
TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA, IMP]
core RNA polymerase II recruiting transcription factor activity [IDA, IMP]

Gene Ontology Cellular Component

nucleus [IDA]

transcriptional preinitiation complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Co-crystal Structure

Interaction directly demonstrated at the atomic level by X-ray crystallography. Also used for NMR or Electron Microscopy (EM) structures. If there is no obvious bait-hit directionality to the interaction involving 3 or more proteins, then the co-crystallized proteins should be listed as a complex.

Publication

Structural studies of a yeast quaternary transcription-initiation complex.

Wang SM, Kim GJ, Gewirth DT

A 96.7 kDa quaternary transcription-factor complex consisting of the conserved core domains of yeast TBP, TFIIA, TFIIB and TATA-box DNA has been assembled from purified components. Crystals of the complex were obtained by the hanging-drop vapor-diffusion method. Native data sets were collected at synchrotron sources. Crystal form I belongs to space group R32, with unit-cell parameters a = b = ... [more]

Acta Crystallogr. D Biol. Crystallogr. Mar. 01, 2001; 57(0);441-4 [Pubmed: 11223526]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TOA1 SUA7	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Costanzo M (2016)	High	-0.1629	BioGRID	1952897

Curated By

BioGRID

Interaction Summary

TOA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

TBP-class protein binding [IDA]TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA, IGI]

Gene Ontology Cellular Component

SUA7

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core binding [IDA, IPI]TBP-class protein binding [IDA, IPI]TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA, IMP]core RNA polymerase II recruiting transcription factor activity [IDA, IMP]

Gene Ontology Cellular Component

Co-crystal Structure

Publication

Structural studies of a yeast quaternary transcription-initiation complex.

Throughput

Related interactions

Curated By

TBP-class protein binding [IDA]
TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA, IGI]

RNA polymerase II core binding [IDA, IPI]
TBP-class protein binding [IDA, IPI]
TBP-class protein binding RNA polymerase II transcription factor activity involved in preinitiation complex assembly [IDA, IMP]
core RNA polymerase II recruiting transcription factor activity [IDA, IMP]