Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Identification of novel Saccharomyces cerevisiae proteins with nuclear export activity: cell cycle-regulated transcription factor ace2p shows cell cycle-independent nucleocytoplasmic shuttling.

Jensen TH, Neville M, Rain JC, McCarthy T, Legrain P, Rosbash M

Nuclear export of proteins containing leucine-rich nuclear export signals (NESs) is mediated by the NES receptor CRM1/Crm1p. We have carried out a yeast two-hybrid screen with Crm1p as a bait. The Crm1p-interacting clones were subscreened for nuclear export activity in a visual assay utilizing the Crm1p-inhibitor leptomycin B (LMB). This approach identified three Saccharomyces cerevisiae proteins not previously known to ... [more]

Mol. Cell. Biol. Nov. 01, 2000; 20(21);8047-58 [Pubmed: 11027275]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CRM1 SGM1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Neuber A (2008)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CRM1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein transporter activity [IDA, IMP]

Gene Ontology Cellular Component

SGM1