DOK2
Gene Ontology Biological Process
LCK
Gene Ontology Biological Process
- B cell receptor signaling pathway [IBA]
- Fc-epsilon receptor signaling pathway [TAS]
- T cell costimulation [TAS]
- T cell differentiation [IMP, ISS]
- T cell receptor signaling pathway [TAS]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [IDA, ISS]
- blood coagulation [TAS]
- cellular response to peptide hormone stimulus [IBA]
- cellular zinc ion homeostasis [IEP, ISS]
- dephosphorylation [IDA, ISS]
- epidermal growth factor receptor signaling pathway [TAS]
- fibroblast growth factor receptor signaling pathway [TAS]
- hemopoiesis [NAS]
- innate immune response [IBA, TAS]
- leukocyte migration [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- peptidyl-tyrosine autophosphorylation [IBA]
- phosphatidylinositol-mediated signaling [TAS]
- platelet activation [TAS]
- positive regulation of T cell activation [IDA, ISS]
- positive regulation of T cell receptor signaling pathway [NAS]
- positive regulation of intrinsic apoptotic signaling pathway [IMP, ISS]
- protein phosphorylation [IDA]
- regulation of cell proliferation [IBA]
- regulation of defense response to virus by virus [TAS]
- regulation of lymphocyte activation [NAS]
- release of sequestered calcium ion into cytosol [ISS]
- response to drug [IDA, ISS]
- transmembrane receptor protein tyrosine kinase signaling pathway [IBA]
- viral process [TAS]
Gene Ontology Molecular Function- ATPase binding [IPI]
- CD4 receptor binding [IPI]
- CD8 receptor binding [IPI]
- SH2 domain binding [IPI, ISS]
- glycoprotein binding [IPI]
- identical protein binding [IPI]
- non-membrane spanning protein tyrosine kinase activity [IBA]
- phosphatidylinositol 3-kinase binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- protein phosphatase binding [IPI]
- protein serine/threonine phosphatase activity [IDA, ISS]
- protein tyrosine kinase activity [EXP, IDA, ISS, TAS]
- ATPase binding [IPI]
- CD4 receptor binding [IPI]
- CD8 receptor binding [IPI]
- SH2 domain binding [IPI, ISS]
- glycoprotein binding [IPI]
- identical protein binding [IPI]
- non-membrane spanning protein tyrosine kinase activity [IBA]
- phosphatidylinositol 3-kinase binding [IPI]
- protein C-terminus binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- protein phosphatase binding [IPI]
- protein serine/threonine phosphatase activity [IDA, ISS]
- protein tyrosine kinase activity [EXP, IDA, ISS, TAS]
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Phospho-tyrosine dependent protein-protein interaction network.
Post-translational protein modifications, such as tyrosine phosphorylation, regulate protein-protein interactions (PPIs) critical for signal processing and cellular phenotypes. We extended an established yeast two-hybrid system employing human protein kinases for the analyses of phospho-tyrosine (pY)-dependent PPIs in a direct experimental, large-scale approach. We identified 292 mostly novel pY-dependent PPIs which showed high specificity with respect to kinases and interacting proteins ... [more]
Throughput
- High Throughput
Additional Notes
- phospho-tyrosine dependent, interaction occurs in the presence of a kinase
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| LCK DOK2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 2549545 |
Curated By
- BioGRID