Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Systematic identification of molecular links between core and candidate genes in breast cancer.

Arroyo R, Sune G, Zanzoni A, Duran-Frigola M, Alcalde V, Stracker TH, Soler-Lopez M, Aloy P

Despite the remarkable progress achieved in the identification of specific genes involved in breast cancer (BC), our understanding of their complex functioning is still limited. In this manuscript, we systematically explore the existence of direct physical interactions between the products of BC core and associated genes. Our aim is to generate a protein interaction network of BC-associated gene products and ... [more]

J. Mol. Biol. Mar. 27, 2015; 427(6);1436-50 [Pubmed: 25640309]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ST14 NOTCH2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	0.9565	BioGRID	3122040

Curated By

BioGRID

Interaction Summary

NOTCH2

Gene Ontology Biological Process

Gene Ontology Molecular Function

ligand-activated RNA polymerase II transcription factor binding transcription factor activity [TAS]protein binding [IPI]receptor activity [NAS]

Gene Ontology Cellular Component

ST14