Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Untethering the TIR1 auxin receptor from the SCF complex increases its stability and inhibits auxin response.

Yu H, Zhang Y, Moss BL, Bargmann BO, Wang R, Prigge M, Nemhauser JL, Estelle M

Plant genomes encode large numbers of F-box proteins (FBPs), the substrate recognition subunit of SKP1-CULLIN-F-box (SCF) ubiquitin ligases. There are ~700 FBPs in Arabidopsis, most of which are uncharacterized. TIR1 is among the best-studied plant FBPs and functions as a receptor for the plant hormone auxin. Here we use a yeast two-hybrid system to identify novel TIR1 mutants with altered ... [more]

Nat Plants Mar. 01, 2015; 1(3); [Pubmed: 26236497]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TIR1 IAA5	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Calderon Villalobos LI (2012)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

TIR1

Gene Ontology Biological Process

Gene Ontology Molecular Function

auxin binding [IDA, IGI]inositol hexakisphosphate binding [IDA]protein binding [IPI]ubiquitin-protein transferase activity [IC, ISS]

Gene Ontology Cellular Component

IAA5