Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A combinatorial TIR1/AFB-Aux/IAA co-receptor system for differential sensing of auxin.

Calderon Villalobos LI, Lee S, De Oliveira C, Ivetac A, Brandt W, Armitage L, Sheard LB, Tan X, Parry G, Mao H, Zheng N, Napier R, Kepinski S, Estelle M

The plant hormone auxin regulates virtually every aspect of plant growth and development. Auxin acts by binding the F-box protein transport inhibitor response 1 (TIR1) and promotes the degradation of the AUXIN/INDOLE-3-ACETIC ACID (Aux/IAA) transcriptional repressors. Here we show that efficient auxin binding requires assembly of an auxin co-receptor complex consisting of TIR1 and an Aux/IAA protein. Heterologous experiments in ... [more]

Unknown Apr. 01, 2012; 0(0); [Pubmed: 22466420]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TIR1 IAA5	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Yu H (2015)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

TIR1

Gene Ontology Biological Process

Gene Ontology Molecular Function

auxin binding [IDA, IGI]inositol hexakisphosphate binding [IDA]protein binding [IPI]ubiquitin-protein transferase activity [IC, ISS]

Gene Ontology Cellular Component

IAA5