KSS1
Gene Ontology Biological Process
- invasive growth in response to glucose limitation [IMP]
- negative regulation of sequence-specific DNA binding transcription factor activity [IMP]
- pheromone-dependent signal transduction involved in conjugation with cellular fusion [IEP, IMP]
- protein phosphorylation [IDA]
- signal transduction involved in filamentous growth [IGI, IMP]
Gene Ontology Molecular Function
STE11
Gene Ontology Biological Process
- MAPK cascade involved in cell wall organization or biogenesis [IGI]
- MAPK cascade involved in osmosensory signaling pathway [IGI, IMP]
- activation of MAPKK activity [IDA, IMP]
- cellular response to heat [IMP]
- invasive growth in response to glucose limitation [IMP]
- osmosensory signaling pathway via Sho1 osmosensor [IGI]
- pheromone-dependent signal transduction involved in conjugation with cellular fusion [IMP]
- protein phosphorylation [IDA, IMP]
- pseudohyphal growth [IMP]
- regulation of transposition, RNA-mediated [IMP]
- signal transduction involved in filamentous growth [IMP]
Gene Ontology Molecular Function
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Protein-protein interactions in the yeast pheromone response pathway: Ste5p interacts with all members of the MAP kinase cascade.
We have used the two-hybrid system of Fields and Song to identify protein-protein interactions that occur in the pheromone response pathway of the yeast Saccharomyces cerevisiae. Pathway components Ste4p, Ste5p, Ste7p, Ste11p, Ste12p, Ste20p, Fus3p and Kss1p were tested in all pairwise combinations. All of the interactions we detected involved at least one member of the MAP kinase cascade that ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| KSS1 STE11 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| KSS1 STE11 | PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay. | High | - | BioGRID | 485979 | |
| KSS1 STE11 | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 160498 | |
| STE11 KSS1 | Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 520688 | |
| KSS1 STE11 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - | |
| STE11 KSS1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID