SPA2
Gene Ontology Biological Process
- bipolar cellular bud site selection [IMP]
- budding cell apical bud growth [IGI, IMP]
- establishment of cell polarity [IMP]
- invasive filamentous growth [IGI]
- mating projection assembly [IGI]
- positive regulation of actin cytoskeleton reorganization [IGI, IMP]
- pseudohyphal growth [IMP]
- regulation of initiation of mating projection growth [IMP]
- regulation of protein localization [IMP]
- regulation of termination of mating projection growth [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SLT2
Gene Ontology Biological Process
- UFP-specific transcription factor mRNA processing involved in endoplasmic reticulum unfolded protein response [IMP]
- barrier septum assembly [IGI]
- endoplasmic reticulum unfolded protein response [IDA, IMP]
- fungal-type cell wall biogenesis [IGI]
- peroxisome degradation [IMP]
- protein phosphorylation [IDA, IMP]
- regulation of cell size [IMP]
- regulation of fungal-type cell wall organization [IGI, IMP]
- regulation of transcription factor import into nucleus [IMP]
- response to acidic pH [IMP]
- signal transduction [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Co-localization
Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.
Publication
Spa2p functions as a scaffold-like protein to recruit the Mpk1p MAP kinase module to sites of polarized growth.
Scaffold proteins play a major role in regulating MAP kinase pathways. In yeast, the Mpk1p-MAP kinase pathway functions to maintain the integrity of the cytoskeleton and the cell wall. In this module, the MEKK Bck1p functions upstream of the MEKs Mkk1p and Mkk2p, which in turn activate the MAP kinase Mpk1p. Mpk1p regulates several nuclear targets, including the transcription factors ... [more]
Throughput
- Low Throughput
Additional Notes
- Mpk1p-GFP and Mkk1p-GFP failed to accumulate at bud tips and bud neck in spa2 or spa2-2 cells
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SPA2 SLT2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 2751640 | |
| SLT2 SPA2 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.296 | BioGRID | 385221 | |
| SLT2 SPA2 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.4071 | BioGRID | 2125918 | |
| SPA2 SLT2 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.1848 | BioGRID | 2148204 | |
| SLT2 SPA2 | Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores. | High | -0.296 | BioGRID | 910056 | |
| SPA2 SLT2 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | High | - | BioGRID | 341371 | |
| SLT2 SPA2 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | Low | - | BioGRID | 160874 | |
| SLT2 SPA2 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | Low | - | BioGRID | 158302 | |
| SLT2 SPA2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID