BAIT
AKAP9
AKAP-9, AKAP350, AKAP450, CG-NAP, HYPERION, LQT11, MU-RMS-40.16A, PPP1R45, PRKA9, YOTIAO
A kinase (PRKA) anchor protein 9
GO Process (10)
GO Function (5)
GO Component (6)
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- cellular response to cAMP [IMP]
- mitotic cell cycle [TAS]
- positive regulation of peptidyl-serine phosphorylation [IMP]
- positive regulation of potassium ion transmembrane transporter activity [IMP]
- regulation of heart rate by cardiac conduction [IMP]
- regulation of membrane repolarization [IMP]
- regulation of ventricular cardiac muscle cell membrane repolarization [IMP]
- synaptic transmission [TAS]
- transport [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
GPC4
K-glypican, RP6-198C21.1
glypican 4
GO Process (10)
GO Function (0)
GO Component (7)
Gene Ontology Biological Process
- anatomical structure morphogenesis [TAS]
- carbohydrate metabolic process [TAS]
- cell proliferation [TAS]
- chondroitin sulfate metabolic process [TAS]
- glycosaminoglycan biosynthetic process [TAS]
- glycosaminoglycan catabolic process [TAS]
- glycosaminoglycan metabolic process [TAS]
- phototransduction, visible light [TAS]
- retinoid metabolic process [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Cellular Component
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Next-generation sequencing to generate interactome datasets.
Next-generation sequencing has not been applied to protein-protein interactome network mapping so far because the association between the members of each interacting pair would not be maintained in en masse sequencing. We describe a massively parallel interactome-mapping pipeline, Stitch-seq, that combines PCR stitching with next-generation sequencing and used it to generate a new human interactome dataset. Stitch-seq is applicable to ... [more]
Nat. Methods Jun. 01, 2011; 8(6);478-80 [Pubmed: 21516116]
Throughput
- High Throughput
Curated By
- BioGRID