BAIT
DYNLL2
DNCL1B, Dlc2, RSPH22
dynein, light chain, LC8-type 2
GO Process (4)
GO Function (1)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
SKAP1
HEL-S-81p, SCAP1, SKAP55
src kinase associated phosphoprotein 1
GO Process (4)
GO Function (6)
GO Component (3)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Next-generation sequencing to generate interactome datasets.
Next-generation sequencing has not been applied to protein-protein interactome network mapping so far because the association between the members of each interacting pair would not be maintained in en masse sequencing. We describe a massively parallel interactome-mapping pipeline, Stitch-seq, that combines PCR stitching with next-generation sequencing and used it to generate a new human interactome dataset. Stitch-seq is applicable to ... [more]
Nat. Methods Jun. 01, 2011; 8(6);478-80 [Pubmed: 21516116]
Throughput
- High Throughput
Curated By
- BioGRID