FHL2
Gene Ontology Biological Process
- androgen receptor signaling pathway [NAS]
- cellular lipid metabolic process [TAS]
- negative regulation of apoptotic process [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of transcription, DNA-templated [NAS]
- response to hormone [IMP]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RFX3
Gene Ontology Biological Process
- cell maturation [ISS]
- cilium assembly [IBA, ISS]
- cilium-dependent cell motility [ISS]
- endocrine pancreas development [ISS]
- epithelial cilium movement involved in determination of left/right asymmetry [ISS]
- negative regulation of transcription, DNA-templated [IDA]
- positive regulation of transcription from RNA polymerase II promoter [ISS]
- positive regulation of transcription, DNA-templated [IDA]
- positive regulation of type B pancreatic cell development [ISS]
- regulation of insulin secretion [ISS]
- regulation of transcription from RNA polymerase II promoter [IBA]
- regulation of transcription, DNA-templated [ISS]
- transcription from RNA polymerase II promoter [TAS]
- type B pancreatic cell differentiation [IBA]
Gene Ontology Molecular Function- DNA binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IBA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [TAS]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [NAS]
- transcription regulatory region DNA binding [IDA]
- DNA binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IBA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [TAS]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [NAS]
- transcription regulatory region DNA binding [IDA]
Gene Ontology Cellular Component
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Next-generation sequencing to generate interactome datasets.
Next-generation sequencing has not been applied to protein-protein interactome network mapping so far because the association between the members of each interacting pair would not be maintained in en masse sequencing. We describe a massively parallel interactome-mapping pipeline, Stitch-seq, that combines PCR stitching with next-generation sequencing and used it to generate a new human interactome dataset. Stitch-seq is applicable to ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| FHL2 RFX3 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 3081199 | |
| RFX3 FHL2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | 2689085 | |
| RFX3 FHL2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | 2701111 | |
| FHL2 RFX3 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID