BAIT
EIF3E
EIF3-P48, EIF3S6, INT6, eIF3-p46
eukaryotic translation initiation factor 3, subunit E
GO Process (7)
GO Function (4)
GO Component (9)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
MAPK1IP1L
C14orf32, MISS, c14_5346
mitogen-activated protein kinase 1 interacting protein 1-like
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Next-generation sequencing to generate interactome datasets.
Next-generation sequencing has not been applied to protein-protein interactome network mapping so far because the association between the members of each interacting pair would not be maintained in en masse sequencing. We describe a massively parallel interactome-mapping pipeline, Stitch-seq, that combines PCR stitching with next-generation sequencing and used it to generate a new human interactome dataset. Stitch-seq is applicable to ... [more]
Nat. Methods Jun. 01, 2011; 8(6);478-80 [Pubmed: 21516116]
Throughput
- High Throughput
Curated By
- BioGRID