BAIT
PDE9A
HSPDE9A2
phosphodiesterase 9A
GO Process (3)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
TFAP4
AP-4, bHLHc41
transcription factor AP-4 (activating enhancer binding protein 4)
GO Process (13)
GO Function (10)
GO Component (2)
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator [IDA]
- negative regulation by host of viral transcription [IDA, NAS]
- negative regulation of DNA binding [IDA]
- negative regulation of cell cycle arrest [IMP]
- negative regulation of cell proliferation [IMP]
- negative regulation of cyclin-dependent protein serine/threonine kinase activity [IDA]
- negative regulation of transcription, DNA-templated [IDA, IMP]
- positive regulation by host of viral transcription [IDA]
- positive regulation of apoptotic process [IDA]
- positive regulation of transcription, DNA-templated [IMP]
- protein complex assembly [IDA]
- regulation of transcription from RNA polymerase II promoter [IBA]
- transcription from RNA polymerase II promoter [IDA]
Gene Ontology Molecular Function- DNA binding [IDA]
- E-box binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IMP]
- protein homodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- transcription coactivator activity [IDA]
- transcription regulatory region DNA binding [IDA]
- DNA binding [IDA]
- E-box binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [IDA]
- histone deacetylase binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [IMP]
- protein homodimerization activity [IPI]
- sequence-specific DNA binding [IDA]
- transcription coactivator activity [IDA]
- transcription regulatory region DNA binding [IDA]
Gene Ontology Cellular Component
Homo sapiens
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Next-generation sequencing to generate interactome datasets.
Next-generation sequencing has not been applied to protein-protein interactome network mapping so far because the association between the members of each interacting pair would not be maintained in en masse sequencing. We describe a massively parallel interactome-mapping pipeline, Stitch-seq, that combines PCR stitching with next-generation sequencing and used it to generate a new human interactome dataset. Stitch-seq is applicable to ... [more]
Nat. Methods Jun. 01, 2011; 8(6);478-80 [Pubmed: 21516116]
Throughput
- High Throughput
Curated By
- BioGRID