BAIT

NECAB2

EFCBP2

N-terminal EF-hand calcium binding protein 2

GO Process (0)

GO Function (1)

GO Component (0)

Gene Ontology Molecular Function

protein binding [IPI]

CRISPR Database VEGA HGNC Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

PREY

CCDC121

hCG_1988995

coiled-coil domain containing 121

GO Process (0)

GO Function (0)

GO Component (0)

CRISPR Database HGNC Alliance of Genome Resources VEGA Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Next-generation sequencing to generate interactome datasets.

Yu H, Tardivo L, Tam S, Weiner E, Gebreab F, Fan C, Svrzikapa N, Hirozane-Kishikawa T, Rietman E, Yang X, Sahalie J, Salehi-Ashtiani K, Hao T, Cusick ME, Hill DE, Roth FP, Braun P, Vidal M

Next-generation sequencing has not been applied to protein-protein interactome network mapping so far because the association between the members of each interacting pair would not be maintained in en masse sequencing. We describe a massively parallel interactome-mapping pipeline, Stitch-seq, that combines PCR stitching with next-generation sequencing and used it to generate a new human interactome dataset. Stitch-seq is applicable to ... [more]

Nat. Methods Jun. 01, 2011; 8(6);478-80 [Pubmed: 21516116]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary