An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances.

Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M

The organization of a cell emerges from the interactions in protein networks. The interactome is critically dependent on the strengths of interactions and the cellular abundances of the connected proteins, both of which span orders of magnitude. However, these aspects have not yet been analyzed globally. Here, we have generated a library of HeLa cell lines expressing 1,125 GFP-tagged proteins ... [more]

Cell Oct. 22, 2015; 163(3);712-23 [Pubmed: 26496610]

Throughput

High Throughput

Additional Notes

interaction detected by quantitative BAC-GFP interactomics (QUBIC)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
UBE2I SUMO1	Affinity Capture-Luminescence Affinity Capture-Luminescence An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.	Trepte P (2015)	Low	-	BioGRID	2751908
UBE2I SUMO1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Ewing RM (2007)	High	0.386	BioGRID	242174
UBE2I SUMO1	Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.	Knipscheer P (2007)	Low	-	BioGRID	1100612
UBE2I SUMO1	Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.	Matic I (2008)	Low	-	BioGRID	1100866
UBE2I SUMO1	Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.	Song J (2009)	Low	-	BioGRID	2497967
UBE2I SUMO1	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Wan C (2015)	High	1	BioGRID	1272884
SUMO1 UBE2I	FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.	Martin SF (2008)	Low	-	BioGRID	905171
UBE2I SUMO1	FRET FRET An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.	Song Y (2011)	Low	-	BioGRID	565956
UBE2I SUMO1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Carbia-Nagashima A (2007)	Low	-	BioGRID	1066758
UBE2I SUMO1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Everett RD (2014)	Low	-	BioGRID	935483
UBE2I SUMO1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Gong L (1997)	Low	-	BioGRID	695850
SUMO1 UBE2I	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Hecker CM (2006)	Low	-	BioGRID	904713
UBE2I SUMO1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Hemelaar J (2004)	Low	-	BioGRID	727175
UBE2I SUMO1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Knipscheer P (2007)	Low	-	BioGRID	673681
SUMO1 UBE2I	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Tatham MH (2003)	Low	-	BioGRID	255608
UBE2I SUMO1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Zilio N (2013)	Low	-	BioGRID	1060673
SUMO1 UBE2I	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Boutell C (2011)	Low	-	BioGRID	685047
SUMO1 UBE2I	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Gong L (1997)	Low	-	BioGRID	695849
SUMO1 UBE2I	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Hecker CM (2006)	Low	-	BioGRID	904674
UBE2I SUMO1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Luck K (2020)	High	-	BioGRID	2708049
UBE2I SUMO1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Minty A (2000)	Low	-	BioGRID	262344
UBE2I SUMO1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Rolland T (2014)	High	-	BioGRID	-
SUMO1 UBE2I	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Shen Z (1996)	Low	-	BioGRID	304555

Curated By

BioGRID

Interaction Summary

UBE2I

Gene Ontology Biological Process

Gene Ontology Molecular Function

RING-like zinc finger domain binding [IPI]SUMO transferase activity [IDA]enzyme binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]transcription factor binding [IPI]ubiquitin protein ligase activity [IBA]ubiquitin protein ligase binding [IBA]ubiquitin-protein transferase activity [TAS]

Gene Ontology Cellular Component

SUMO1