An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances.

Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M

The organization of a cell emerges from the interactions in protein networks. The interactome is critically dependent on the strengths of interactions and the cellular abundances of the connected proteins, both of which span orders of magnitude. However, these aspects have not yet been analyzed globally. Here, we have generated a library of HeLa cell lines expressing 1,125 GFP-tagged proteins ... [more]

Cell Oct. 22, 2015; 163(3);712-23 [Pubmed: 26496610]

Throughput

High Throughput

Additional Notes

interaction detected by quantitative BAC-GFP interactomics (QUBIC)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PDK3 CCT2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Buljan M (2020)	High	201.3158	BioGRID	3484003

Curated By

BioGRID

Interaction Summary

PDK3

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [IDA]protein binding [IPI]protein kinase activity [IDA]protein serine/threonine kinase activity [IDA]pyruvate dehydrogenase (acetyl-transferring) kinase activity [IDA]

Gene Ontology Cellular Component

CCT2

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]ubiquitin protein ligase binding [IPI]unfolded protein binding [NAS]