ADR1
Gene Ontology Biological Process
- cellular response to oleic acid [IPI]
- chromatin organization [IDA]
- chromatin organization involved in regulation of transcription [IGI, IMP, IPI]
- negative regulation of chromatin silencing [IDA]
- peroxisome organization [IMP]
- positive regulation of ethanol catabolic process by positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of fatty acid beta-oxidation by positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of peroxisome organization by positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription from RNA polymerase II promoter by oleic acid [IMP]
- positive regulation of transcription from RNA polymerase II promoter in response to ethanol [IMP]
Gene Ontology Molecular Function- RNA polymerase II activating transcription factor binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IPI]
- TFIIB-class transcription factor binding [IDA]
- TFIID-class transcription factor binding [IDA]
- sequence-specific DNA binding [IDA]
- RNA polymerase II activating transcription factor binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity [IDA]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IPI]
- TFIIB-class transcription factor binding [IDA]
- TFIID-class transcription factor binding [IDA]
- sequence-specific DNA binding [IDA]
TAF1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
ADR1-mediated transcriptional activation requires the presence of an intact TFIID complex.
The yeast transcriptional activator ADR1, which is required for ADH2 and other genes' expression, contains four transactivation domains (TADs). While previous studies have shown that these TADs act through GCN5 and ADA2, and presumably TFIIB, other factors are likely to be involved in ADR1 function. In this study, we addressed the question of whether TFIID is also required for ADR1 ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ADR1 TAF1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID