Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

Using an in situ proximity ligation assay to systematically profile endogenous protein-protein interactions in a pathway network.

Chen TC, Lin KT, Chen CH, Lee SA, Lee PY, Liu YW, Kuo YL, Wang FS, Lai JM, Huang CY

Signal transduction pathways in the cell require protein-protein interactions (PPIs) to respond to environmental cues. Diverse experimental techniques for detecting PPIs have been developed. However, the huge amount of PPI data accumulated from various sources poses a challenge with respect to data reliability. Herein, we collected âˆ¼ 700 primary antibodies and employed a highly sensitive and specific technique, an in ... [more]

J. Proteome Res. Dec. 05, 2014; 13(12);5339-46 [Pubmed: 25241761]

Throughput

High Throughput

Additional Notes

in situ PLA

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
PTPRR MAPK1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2015)	High	0.9023	BioGRID	1193680
PTPRR MAPK1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2017)	High	0.9446	BioGRID	2252753
PTPRR MAPK1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	0.9526	BioGRID	3114560
PTPRR MAPK1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Kumar P (2017)	High	-	BioGRID	-
PTPRR MAPK1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	St-Denis N (2016)	High	-	BioGRID	-
PTPRR MAPK1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Zuniga A (1999)	Low	-	BioGRID	-
MAPK1 PTPRR	Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.	Zuniga A (1999)	Low	-	BioGRID	604680
PTPRR MAPK1	Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.	Zuniga A (1999)	Low	-	BioGRID	604682
PTPRR MAPK1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Pulido R (1998)	Low	-	BioGRID	-
PTPRR MAPK1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Zuniga A (1999)	Low	-	BioGRID	-
MAPK1 PTPRR	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Zuniga A (1999)	Low	-	BioGRID	-
MAPK1 PTPRR	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Luck K (2020)	High	-	BioGRID	2704685

Curated By

BioGRID

Interaction Summary

PTPRR

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein kinase binding [ISS]protein tyrosine phosphatase activity [TAS]transmembrane receptor protein tyrosine phosphatase activity [TAS]

Gene Ontology Cellular Component

MAPK1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II carboxy-terminal domain kinase activity [ISS]phosphatase binding [IPI]protein binding [IPI]protein serine/threonine kinase activity [IDA]