BAIT

SIW14

OCA3, putative tyrosine protein phosphatase SIW14, L000004216, YNL032W
Tyrosine phosphatase involved in actin organization and endocytosis; localized to the cytoplasm
GO Process (3)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

VIP1

inositol polyphosphate kinase VIP1, L000004547, YLR410W
Inositol hexakisphosphate and inositol heptakisphosphate kinase; inositol heptakisphosphate (IP7) production is important for phosphate signaling; involved in cortical actin cytoskeleton function, and invasive pseudohyphal growth analogous to S. pombe asp1; inositol hexakisphosphate is also known as IP6
Saccharomyces cerevisiae (S288c)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

A novel inositol pyrophosphate phosphatase in Saccharomyces cerevisiae: Siw14 selectively cleaves the β-phosphate from 5-diphosphoinositol pentakisphosphate (5PP-IP5).

Steidle EA, Chong LS, Wu M, Crooke E, Fiedler D, Resnick AC, Rolfes RJ

Inositol pyrophosphates are high energy signaling molecules involved in cellular processes such as energetic metabolism, telomere maintenance, stress responses, vesicle trafficking, and can mediate protein phosphorylation. While the inositol kinases underlying inositol pyrophosphate biosynthesis and are well characterized, the phosphatases that selectively regulate their cellular pools are not fully described. The DIPP (diphosphoinositol phosphate phosphohydrolase) enzymes of the Nudix protein ... [more]

J. Biol. Chem. Jan. 31, 2016; 0(0); [Pubmed: 26828065]

Throughput

  • Low Throughput

Ontology Terms

  • phenotype: chemical compound accumulation (APO:0000095)

Additional Notes

  • Figure 8
  • double mutants show increase in inositol phosphates

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
VIP1 SIW14
Positive Genetic
Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

High0.1913BioGRID
1912360
VIP1 SIW14
Positive Genetic
Positive Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a less severe fitness defect than expected under a given condition. This term is reserved for high or low throughput studies with scores.

High3.8861BioGRID
325096

Curated By

  • BioGRID