BAIT

FUS6

ARABIDOPSIS THALIANA FUSCA 6, ATFUS6, ATSK31, CONSTITUTIVE PHOTOMORPHOGENIC 11, COP11, COP9 SIGNALOSOME SUBUNIT 1, CSN1, EMB78, EMBRYO DEFECTIVE 78, FUSCA 6, SHAGGY-LIKE KINASE 31, SK31, AT3G61140
COP9 signalosome complex subunit 1
Arabidopsis thaliana (Columbia)
PREY

COP8

ATS4, CONSTITUTIVE PHOTOMORPHOGENIC 14, CONSTITUTIVE PHOTOMORPHOGENIC 8, COP14, COP9 SIGNALOSOME SUBUNIT 4, CSN4, EMB134, EMBRYO DEFECTIVE 134, FUS4, FUS8, FUSCA 4, FUSCA 8, MBD2.17, MBD2_17, AT5G42970
COP9 signalosome complex subunit 4
GO Process (3)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Regulation of COP1 nuclear localization by the COP9 signalosome via direct interaction with CSN1.

Wang X, Li W, Piqueras R, Cao K, Deng XW, Wei N

COP1 and COP9 signalosome (CSN) are key regulators of plant light responses and development. Deficiency in either COP1 or CSN causes a constitutive photomorphogenic phenotype. Through coordinated actions of nuclear- and cytoplasmic-localization signals, COP1 can respond to light signals by differentially partitions between nuclear and cytoplasmic compartments. Previous genetic analysis in Arabidopsis indicated that the nuclear localization of COP1 requires ... [more]

Plant J. May. 01, 2009; 58(4);655-67 [Pubmed: 19175768]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
COP8 FUS6
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High0.9763BioGRID
2911043
COP8 FUS6
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BAR
-
COP8 FUS6
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID