BAIT

COP8

ATS4, CONSTITUTIVE PHOTOMORPHOGENIC 14, CONSTITUTIVE PHOTOMORPHOGENIC 8, COP14, COP9 SIGNALOSOME SUBUNIT 4, CSN4, EMB134, EMBRYO DEFECTIVE 134, FUS4, FUS8, FUSCA 4, FUSCA 8, MBD2.17, MBD2_17, AT5G42970
COP9 signalosome complex subunit 4
GO Process (3)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

FUS6

ARABIDOPSIS THALIANA FUSCA 6, ATFUS6, ATSK31, CONSTITUTIVE PHOTOMORPHOGENIC 11, COP11, COP9 SIGNALOSOME SUBUNIT 1, CSN1, EMB78, EMBRYO DEFECTIVE 78, FUSCA 6, SHAGGY-LIKE KINASE 31, SK31, AT3G61140
COP9 signalosome complex subunit 1
Arabidopsis thaliana (Columbia)

Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

A Pan-plant Protein Complex Map Reveals Deep Conservation and Novel Assemblies.

McWhite CD, Papoulas O, Drew K, Cox RM, June V, Dong OX, Kwon T, Wan C, Salmi ML, Roux SJ, Browning KS, Chen ZJ, Ronald PC, Marcotte EM

Plants are foundational for global ecological and economic systems, but most plant proteins remain uncharacterized. Protein interaction networks often suggest protein functions and open new avenues to characterize genes and proteins. We therefore systematically determined protein complexes from 13 plant species of scientific and agricultural importance, greatly expanding the known repertoire of stable protein complexes in plants. By using co-fractionation ... [more]

Cell Mar. 16, 2020; (); [Pubmed: 32191846]

Quantitative Score

  • 0.976331807 [CF-MS score]

Throughput

  • High Throughput

Additional Notes

  • Co-fractionation MS (CF-MS) was carried out to identify high confidence protein interactions having an interaction score above a 10% FDR threshold (CF-MS score >= 0.509).
  • Interactor A: Orthogroup ENOG411DU82, Annotation: COP9 signalosome complex subunit
  • Interactor B: Orthogroup ENOG411DXM6, Annotation: cop9 signalosome complex subunit

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
FUS6 COP8
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
COP8 FUS6
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BAR
-
COP8 FUS6
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID