GLIS3
Gene Ontology Biological Process
Gene Ontology Cellular Component
SUFU
Gene Ontology Biological Process
- multicellular organismal development [TAS]
- negative regulation of osteoblast differentiation [TAS]
- negative regulation of sequence-specific DNA binding transcription factor activity [TAS]
- negative regulation of smoothened signaling pathway [TAS]
- negative regulation of transcription factor import into nucleus [TAS]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- proteolysis [TAS]
- regulation of transcription, DNA-templated [TAS]
- signal transduction [TAS]
- skeletal system development [TAS]
Gene Ontology Molecular Function
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
HECT E3 Ubiquitin Ligase Itch Functions as a Novel Negative Regulator of Gli-Similar 3 (Glis3) Transcriptional Activity.
The transcription factor Gli-similar 3 (Glis3) plays a critical role in the generation of pancreatic ss cells and the regulation insulin gene transcription and has been implicated in the development of several pathologies, including type 1 and 2 diabetes and polycystic kidney disease. However, little is known about the proteins and posttranslational modifications that regulate or mediate Glis3 transcriptional activity. ... [more]
Throughput
- Low Throughput
Additional Notes
- Figure 1
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SUFU GLIS3 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| SUFU GLIS3 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID