SUFU
Gene Ontology Biological Process
- multicellular organismal development [TAS]
- negative regulation of osteoblast differentiation [TAS]
- negative regulation of sequence-specific DNA binding transcription factor activity [TAS]
- negative regulation of smoothened signaling pathway [TAS]
- negative regulation of transcription factor import into nucleus [TAS]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- proteolysis [TAS]
- regulation of transcription, DNA-templated [TAS]
- signal transduction [TAS]
- skeletal system development [TAS]
Gene Ontology Molecular Function
GLIS3
Gene Ontology Biological Process
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Modulation of the transactivation function and stability of Krueppel-like zinc finger protein Gli-similar 3 (Glis3) by Suppressor of Fused.
Glis3 is a member of the Glis subfamily of Krueppel-like zinc finger transcription factors. Recently, Glis3 has been linked to both type I and type II diabetes and shown to positively regulate insulin gene expression. In this study, we have identified a region within the N terminus of Glis3 that shares high levels of homology with the Cubitus interruptus (Ci)/Gli ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| GLIS3 SUFU | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | 1510360 | |
| SUFU GLIS3 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | - |
Curated By
- BioGRID