An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Epsin is required for Dishevelled stability and Wnt signalling activation in colon cancer development.

Chang B, Tessneer KL, McManus J, Liu X, Hahn S, Pasula S, Wu H, Song H, Chen Y, Cai X, Dong Y, Brophy ML, Rahman R, Ma JX, Xia L, Chen H

Uncontrolled canonical Wnt signalling supports colon epithelial tumour expansion and malignant transformation. Understanding the regulatory mechanisms involved is crucial for elucidating the pathogenesis of and will provide new therapeutic targets for colon cancer. Epsins are ubiquitin-binding adaptor proteins upregulated in several human cancers; however, the involvement of epsins in colon cancer is unknown. Here we show that loss of intestinal ... [more]

Nat Commun Apr. 15, 2015; 6(0);6380 [Pubmed: 25871009]

Throughput

Low Throughput

Additional Notes

Figure S2

Curated By

BioGRID

Interaction Summary

EPN1

Gene Ontology Biological Process

Gene Ontology Cellular Component

FZD8

Gene Ontology Biological Process

Gene Ontology Molecular Function

G-protein coupled receptor activity [IBA]PDZ domain binding [IPI]Wnt-activated receptor activity [TAS]Wnt-protein binding [IBA]protein binding [IPI]ubiquitin protein ligase binding [IPI]