An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Control of APC/C-dependent ubiquitin chain elongation by reversible phosphorylation.

Craney A, Kelly A, Jia L, Fedrigo I, Yu H, Rape M

Most metazoan E3 ligases contain a signature RING domain that promotes the transfer of ubiquitin from the active site of E2 conjugating enzymes to lysine residues in substrates. Although these RING-E3s depend on E2 enzymes for catalysis, how they turn on their E2s at the right time and place remains poorly understood. Here we report a phosphorylation-dependent mechanism that ensures ... [more]

Proc. Natl. Acad. Sci. U.S.A. Feb. 09, 2016; 113(6);1540-5 [Pubmed: 26811472]

Throughput

Low Throughput

Additional Notes

Figure 1

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CDC20 TCP1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Fogeron ML (2013)	High	-	BioGRID	-
CDC20 TCP1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Kelly A (2014)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CDC20

Gene Ontology Biological Process

Gene Ontology Molecular Function

enzyme binding [IPI]protein C-terminus binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

TCP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]protein binding [IPI]ubiquitin protein ligase binding [IPI]