BAIT

POL2

DUN2, DNA polymerase epsilon catalytic subunit, L000001461, YNL262W
Catalytic subunit of DNA polymerase (II) epsilon; a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; participates in leading-strand synthesis during DNA replication; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p
Saccharomyces cerevisiae (S288c)
PREY

CHK1

serine/threonine protein kinase CHK1, L000004861, YBR274W
Serine/threonine kinase and DNA damage checkpoint effector; mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase
Kinome Project (Sc)
GO Process (3)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Genetic Networks Required to Coordinate Chromosome Replication by DNA Polymerases α, δ, and ε in Saccharomyces cerevisiae.

Dubarry M, Lawless C, Banks AP, Cockell S, Lydall D

Three major DNA polymerases replicate the linear eukaryotic chromosomes. DNA polymerase α-primase (Pol α) and DNA polymerase δ (Pol δ) replicate the lagging-strand and Pol α and DNA polymerase ε (Pol ε) the leading-strand. To identify factors affecting coordination of DNA replication, we have performed genome-wide quantitative fitness analyses of budding yeast cells containing defective polymerases. We combined temperature-sensitive mutations ... [more]

G3 (Bethesda) Oct. 01, 2015; 5(10);2187-97 [Pubmed: 26297725]

Throughput

  • High Throughput

Ontology Terms

  • vegetative growth (APO:0000106)

Additional Notes

  • Table S2
  • genome knock-out and DAmP collections used to create double mutants
  • pol2-12 allele
  • quantitative fitness analysis performed on double mutants constructed via SGA technique

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
POL2 CHK1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.6648BioGRID
2012175

Curated By

  • BioGRID