BAIT

ARRB1

ARB1, ARR1
arrestin, beta 1
GO Process (22)
GO Function (9)
GO Component (11)
Homo sapiens
PREY

DRD4

D4DR
dopamine receptor D4
GO Process (24)
GO Function (8)
GO Component (3)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Characterization of the interaction between the dopamine D4 receptor, KLHL12 and β-arrestins.

Skieterska K, Shen A, Clarisse D, Rondou P, Borroto-Escuela DO, Lintermans B, Fuxe K, Xiang YK, Van Craenenbroeck K

Dopamine receptors are G protein-coupled receptors involved in regulation of cognition, learning, movement and endocrine signaling. The action of G protein-coupled receptors is highly regulated by multifunctional proteins, such as β-arrestins which can control receptor desensitization, ubiquitination and signaling. Previously, we have reported that β-arrestin 2 interacts with KLHL12, a BTB-Kelch protein which functions as an adaptor in a Cullin3-based ... [more]

Cell. Signal. Aug. 01, 2016; 28(8);1001-14 [Pubmed: 27155323]

Throughput

  • Low Throughput

Curated By

  • BioGRID